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K430040

Invitrogen™ pBAD TOPO™ TA Expression Kit

Manufacturer: Fischer Scientific

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Pack Size	SKU	Availability	Price
Each of 1	K430040-Each-of-1	In Stock	₹ 1,69,066.56

K430040 - Each of 1

₹ 1,69,066.56

In Stock

Quantity

Base Price: ₹ 1,69,066.56

GST (18%): ₹ 30,431.981

Total Price: ₹ 1,99,498.541

Constitutive or Inducible System

Inducible

Promoter

araBAD

Selection Agent (Eukaryotic)

None

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Cleavage

EK (Enterokinase) Recognition Site

Expression Mechanism

Cell-Based Expression

Cloning Method

TOPO-TA

Vector

pBAD

Inducing Agent

Arabinose

Product Type

TA Expression Kit

Content And Storage

Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194E. colistab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (exceptTaqpolymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194E. colistab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

Bacterial or Yeast Strain

LMG194, TOP10

Expression System

E. coli

Protein Tag

V5 Epitope Tag

Quantity

40 Reactions

Product Line

TOPO™

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Description

Linearized, topoisomerase I-activated vector for 5-minute cloning of Taq amplified PCR products araBAD promoter for tightly regulated expression in E
coli V5 epitope tag for detection with Anti-V5 Antibody C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody Enterokinase cleavage site for removal of N-terminal leader peptide Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Fischer Scientific

K430040

Constitutive or Inducible System:

Inducible

Promoter:

araBAD

Selection Agent (Eukaryotic):

None

Antibiotic Resistance Bacterial:

Ampicillin (AmpR)

Cleavage:

EK (Enterokinase) Recognition Site

Expression Mechanism:

Cell-Based Expression

Cloning Method:

TOPO-TA

Vector:

pBAD

Inducing Agent:

Arabinose

Product Type:

TA Expression Kit

Content And Storage:

Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194E. colistab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (exceptTaqpolymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194E. colistab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

Bacterial or Yeast Strain:

LMG194, TOP10

Expression System:

E. coli

Protein Tag:

V5 Epitope Tag

Quantity:

40 Reactions

Product Line:

TOPO™

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K4377

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Promoter:

Selection Agent (Eukaryotic):

Antibiotic Resistance Bacterial:

Cleavage:

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Constitutive or Inducible System:

Promoter:

Selection Agent (Eukaryotic):

Antibiotic Resistance Bacterial:

Cleavage:

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Expression System:

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Invitrogen™

K440001

Constitutive or Inducible System:

Inducible

Promoter:

Trc, lacO

Selection Agent (Eukaryotic):

None

Antibiotic Resistance Bacterial:

Ampicillin (AmpR)

Cleavage:

EK (Enterokinase) Recognition Site

Expression Mechanism:

Cell-Based Expression

Cloning Method:

TOPO-TA

Vector:

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Inducing Agent:

IPTG

Product Type:

TA Expression Kit

Content And Storage:

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Bacterial or Yeast Strain:

TOP10

Expression System:

E. coli

Protein Tag:

His Tag (6x), c-Myc Epitope Tag

Quantity:

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Product Line:

TOPO™

Invitrogen™ pBAD TOPO™ TA Expression Kit

Select a Size

Select a Size

Properties

Constitutive or Inducible System

Promoter

Selection Agent (Eukaryotic)

Antibiotic Resistance Bacterial

Cleavage

Expression Mechanism

Cloning Method

Vector

Inducing Agent

Product Type

Content And Storage

Bacterial or Yeast Strain

Expression System

Protein Tag

Quantity

Product Line

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Description

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Fischer Scientific

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