K35920
Invitrogen™ Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K35920-Each-of-1 | In Stock | ₹ 2,07,483.00 |
K35920 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,07,483.00
GST (18%): ₹ 37,346.94
Total Price: ₹ 2,44,829.94
Constitutive or Inducible System
Constitutive
Promoter
CMV
Reporter Gene
GFP (EmGFP)
Content And Storage
Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competentE. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competentE. coliat -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method
TOPO-TA
Vector
pcDNA
For Use With (Application)
Reporter Assays, Subcellular Localization
Delivery Type
Transfection
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Blasticidin
Protein Tag
GFP (EmGFP)
Quantity
20 reactions
Product Line
Gateway™, TOPO™, Vivid Colors™, pcDNA™
Related Products
Description
- Powerful TOPO cloning vectors contain Emerald Green Fluorescent Protein (EmGFP) or Yellow Fluorescent Protein (YFP) for simple, noninvasive detection of recombinant protein Both FPs have been humanized for optimal mammalian expression Topoisomerase I for one-step, 5-minute TOPO cloning of PCR-amplified gene of interest CMV promoter for high-level expression of recombinant fluorescent fusion protein Ability to fuse EmGFP or YFP to N- or C-terminus of protein Bsd resistance marker for rapid selection of stable cell lines Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Cloning, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g
- GFP) Assays, Fluorescent Protein Assays (GFP)/Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: GFP (EmGFP)
Content And Storage: Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competentE. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competentE. coliat -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pcDNA
For Use With (Application): Reporter Assays, Subcellular Localization
Delivery Type: Transfection
Product Type: Mammalian Expression Vector
Selection Agent (Eukaryotic): Blasticidin
Protein Tag: GFP (EmGFP)
Quantity: 20 reactions
Product Line: Gateway™, TOPO™, Vivid Colors™, pcDNA™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
GFP (EmGFP)
Content And Storage:
Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competentE. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competentE. coliat -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pcDNA
For Use With (Application):
Reporter Assays, Subcellular Localization
Delivery Type:
Transfection
Product Type:
Mammalian Expression Vector
Selection Agent (Eukaryotic):
Blasticidin
Protein Tag:
GFP (EmGFP)
Quantity:
20 reactions
Product Line:
Gateway™, TOPO™, Vivid Colors™, pcDNA™
Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: __
Content And Storage: pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen
Cloning Method: Gateway
Vector: pLenti
For Use With (Application): Viral Expression
Delivery Type: Lentiviral
Product Type: Lentiviral Expression System
Selection Agent (Eukaryotic): Blasticidin
Protein Tag: V5 Epitope Tag
Quantity: 1 kit
Product Line: Gateway™, ViraPower™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
__
Content And Storage:
pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen
Cloning Method:
Gateway
Vector:
pLenti
For Use With (Application):
Viral Expression
Delivery Type:
Lentiviral
Product Type:
Lentiviral Expression System
Selection Agent (Eukaryotic):
Blasticidin
Protein Tag:
V5 Epitope Tag
Quantity:
1 kit
Product Line:
Gateway™, ViraPower™
Constitutive or Inducible System: Inducible
Promoter: araBAD
Reporter Gene: __
Content And Storage: Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pBAD
For Use With (Application): __
Delivery Type: __
Product Type: Lentiviral Expression Kit
Selection Agent (Eukaryotic): None
Protein Tag: His Tag (6x), V5 Epitope Tag, Thioredoxin
Quantity: 20 reactions
Product Line: TOPO™, ThioFusion™
Constitutive or Inducible System:
Inducible
Promoter:
araBAD
Reporter Gene:
__
Content And Storage:
Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pBAD
For Use With (Application):
__
Delivery Type:
__
Product Type:
Lentiviral Expression Kit
Selection Agent (Eukaryotic):
None
Protein Tag:
His Tag (6x), V5 Epitope Tag, Thioredoxin
Quantity:
20 reactions
Product Line:
TOPO™, ThioFusion™
Constitutive or Inducible System: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
For Use With (Application):
__
Delivery Type:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Protein Tag:
__
Quantity:
__
Product Line:
__