K440001
Invitrogen™ pTrcHis2 TOPO™ TA Expression Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K440001-Each-of-1 | In Stock | ₹ 91,035.84 |
K440001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 91,035.84
GST (18%): ₹ 16,386.451
Total Price: ₹ 1,07,422.291
Constitutive or Inducible System
Inducible
Promoter
Trc, lacO
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Cloning Method
TOPO-TA
Product Line
TOPO™
Inducing Agent
IPTG
Product Type
TA Expression Kit
Content And Storage
The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain
TOP10
Expression System
E. coli
Protein Tag
His Tag (6x), c-Myc Epitope Tag
Vector
pTrc
Related Products
Description
- Vector includes minicistron element for enhanced translation efficiency of eukaryotic proteins in E
- coli Vector provided linearized and activated with topoisomerase I for 5-minute TOPO Cloning with >85% cloning efficiency N-terminal Xpress epitope for detection with Anti-Xpress Antibody N-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-HisG Antibody Enterokinase cleavage site for efficient removal of N-terminal fusion tags pTrcHis2-TOPO™ C-terminal c-myc epitope for detection with Anti- myc Antibody C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody Bacterial Expression/Protein Expression/Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Inducible
Promoter: Trc, lacO
Selection Agent (Eukaryotic): None
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Expression Mechanism: Cell-Based Expression
Cloning Method: TOPO-TA
Product Line: TOPO™
Inducing Agent: IPTG
Product Type: TA Expression Kit
Content And Storage: The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: TOP10
Expression System: E. coli
Protein Tag: His Tag (6x), c-Myc Epitope Tag
Vector: pTrc
Constitutive or Inducible System:
Inducible
Promoter:
Trc, lacO
Selection Agent (Eukaryotic):
None
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Cleavage:
EK (Enterokinase) Recognition Site
Expression Mechanism:
Cell-Based Expression
Cloning Method:
TOPO-TA
Product Line:
TOPO™
Inducing Agent:
IPTG
Product Type:
TA Expression Kit
Content And Storage:
The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain:
TOP10
Expression System:
E. coli
Protein Tag:
His Tag (6x), c-Myc Epitope Tag
Vector:
pTrc
Constitutive or Inducible System: Inducible
Promoter: Trc, lacO
Selection Agent (Eukaryotic): None
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Expression Mechanism: Cell-Based Expression
Cloning Method: TOPO-TA
Product Line: TOPO™
Inducing Agent: IPTG
Product Type: TA Expression Kit
Content And Storage: The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: TOP10
Expression System: E. coli
Protein Tag: His Tag (6x), Xpress Epitope Tag
Vector: pTrc
Constitutive or Inducible System:
Inducible
Promoter:
Trc, lacO
Selection Agent (Eukaryotic):
None
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Cleavage:
EK (Enterokinase) Recognition Site
Expression Mechanism:
Cell-Based Expression
Cloning Method:
TOPO-TA
Product Line:
TOPO™
Inducing Agent:
IPTG
Product Type:
TA Expression Kit
Content And Storage:
The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain:
TOP10
Expression System:
E. coli
Protein Tag:
His Tag (6x), Xpress Epitope Tag
Vector:
pTrc
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Product Line: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Vector: __
Constitutive or Inducible System:
__
Promoter:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
__
Cleavage:
__
Expression Mechanism:
__
Cloning Method:
__
Product Line:
__
Inducing Agent:
__
Product Type:
__
Content And Storage:
__
Bacterial or Yeast Strain:
__
Expression System:
__
Protein Tag:
__
Vector:
__
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Product Line: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Vector: __
Constitutive or Inducible System:
__
Promoter:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
__
Cleavage:
__
Expression Mechanism:
__
Cloning Method:
__
Product Line:
__
Inducing Agent:
__
Product Type:
__
Content And Storage:
__
Bacterial or Yeast Strain:
__
Expression System:
__
Protein Tag:
__
Vector:
__