Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K37001-Each-of-1 | In Stock | ₹ 1,06,436.64 |
K37001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,06,436.64
GST (18%): ₹ 19,158.595
Total Price: ₹ 1,25,595.235
Constitutive or Inducible System
Inducible
Promoter
araBAD
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Cloning Method
TOPO-TA
Vector
pBAD
Inducing Agent
Arabinose
Product Type
Lentiviral Expression Kit
Content And Storage
Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain
TOP10
Expression System
E. coli
Protein Tag
His Tag (6x), V5 Epitope Tag, Thioredoxin
Quantity
20 reactions
Product Line
TOPO™, ThioFusion™
Related Products
Description
- Description pBAD/Thio-TOPO vector encodes His-Patch thioredoxin as N-terminal fusion partner Thioredoxin fusion can significantly increase solubility of many difficult-to-express proteins and improve yield of protein production Vector features araBAD promoter for tightly regulated expression in E
- coli I-activated vector for 5-minute TOPO Cloning of Taq -amplified PCR products C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with aAnti-His(C-term) Antibody C-terminal V5 epitope for detection with an Anti-V5 Antibody Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMaxEnterokinase Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Inducible
Promoter: araBAD
Selection Agent (Eukaryotic): None
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Expression Mechanism: Cell-Based Expression
Cloning Method: TOPO-TA
Vector: pBAD
Inducing Agent: Arabinose
Product Type: Lentiviral Expression Kit
Content And Storage: Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: TOP10
Expression System: E. coli
Protein Tag: His Tag (6x), V5 Epitope Tag, Thioredoxin
Quantity: 20 reactions
Product Line: TOPO™, ThioFusion™
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Vector: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Vector: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Quantity: __
Product Line: __
