K89020

Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K89020-Each-of-1 In Stock ₹ 96,169.44

K89020 - Each of 1

₹ 96,169.44

In Stock

Quantity

1

Base Price: ₹ 96,169.44

GST (18%): ₹ 17,310.499

Total Price: ₹ 1,13,479.939

Promoter

OplE2

Content And Storage

Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot™ box contains high-efficiency, single-use, 50 μl aliquots of chemically competent TOP10E. colicells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

Protein Tag

His Tag (6x), V5 Epitope Tag

Quantity

20 reactions

Product Line

InsectSelect™, TOPO™

Product Type

TOPO TA Expression Kit

Protein Tag Position (to your gene)

C-terminal

Cloning Method

TOPO-TA

Vector

pIB, TOPO-TA Cloning Vectors

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Description

  • Description The OpIE 2 promoter for constitutive expression Includes Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification using nickel-chelating resin Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Promoter:
OplE2

Content And Storage:
Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot™ box contains high-efficiency, single-use, 50 μl aliquots of chemically competent TOP10E. colicells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

Protein Tag:
His Tag (6x), V5 Epitope Tag

Quantity:
20 reactions

Product Line:
InsectSelect™, TOPO™

Product Type:
TOPO TA Expression Kit

Protein Tag Position (to your gene):
C-terminal

Cloning Method:
TOPO-TA

Vector:
pIB, TOPO-TA Cloning Vectors

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