K483001
Invitrogen™ pGlow TOPO™ TA Expression Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K483001-Each-of-1 | In Stock | ₹ 1,00,926.00 |
K483001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,00,926.00
GST (18%): ₹ 18,166.68
Total Price: ₹ 1,19,092.68
Delivery Type
Transfection
Product Type
TOPO TA Expression Kit
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
Untagged
Quantity
20 reactions
Product Line
TOPO™
Promoter
None (Promoterless)
Reporter Gene
GFP (Cycle 3)
Content And Storage
pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method
TOPO-TA
Vector
pGlow, TOPO-TA Vectors
For Use With (Application)
Reporter Assays, Clone your own Promoter
Related Products
Description
- Cycle 3 GFP is exellent for noninvasive analysis of promoter elements within intact, living cells Fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g
- GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
Compare Similar Items
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Delivery Type: Transfection
Product Type: TOPO TA Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: Untagged
Quantity: 20 reactions
Product Line: TOPO™
Promoter: None (Promoterless)
Reporter Gene: GFP (Cycle 3)
Content And Storage: pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pGlow, TOPO-TA Vectors
For Use With (Application): Reporter Assays, Clone your own Promoter
Delivery Type:
Transfection
Product Type:
TOPO TA Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
Untagged
Quantity:
20 reactions
Product Line:
TOPO™
Promoter:
None (Promoterless)
Reporter Gene:
GFP (Cycle 3)
Content And Storage:
pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pGlow, TOPO-TA Vectors
For Use With (Application):
Reporter Assays, Clone your own Promoter
Delivery Type: __
Product Type: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Protein Tag:
__
Quantity:
__
Product Line:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
For Use With (Application):
__
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 Reactions
Product Line: TOPO™, pcDNA™
Promoter: CMV
Reporter Gene: __
Content And Storage: Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method: Directional TOPO
Vector: Directional TOPO Vectors, pcDNA
For Use With (Application): Constitutive Expression
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
20 Reactions
Product Line:
TOPO™, pcDNA™
Promoter:
CMV
Reporter Gene:
__
Content And Storage:
Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method:
Directional TOPO
Vector:
Directional TOPO Vectors, pcDNA
For Use With (Application):
Constitutive Expression
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 40 Reactions
Product Line: TOPO™, pcDNA™
Promoter: CMV
Reporter Gene: __
Content And Storage: 2 × Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)2 × Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method: Directional TOPO
Vector: Directional TOPO Vectors, pcDNA
For Use With (Application): Constitutive Expression
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
40 Reactions
Product Line:
TOPO™, pcDNA™
Promoter:
CMV
Reporter Gene:
__
Content And Storage:
2 × Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)2 × Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method:
Directional TOPO
Vector:
Directional TOPO Vectors, pcDNA
For Use With (Application):
Constitutive Expression