K482001
Invitrogen™ CT-GFP Fusion TOPO™ Expression Kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K482001-Each-of-1 | In Stock | ₹ 1,04,554.32 |
K482001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,04,554.32
GST (18%): ₹ 18,819.778
Total Price: ₹ 1,23,374.098
Constitutive or Inducible System
Constitutive
Promoter
CMV
Reporter Gene
GFP (Cycle 3)
Content And Storage
CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Cloning Method
TOPO-TA
Vector
TOPO-TA Vectors
For Use With (Application)
Reporter Assays
Delivery Type
Transfection
Product Type
TOPO Expression Kit
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
GFP (Cycle 3)
Quantity
20 reactions
Product Line
TOPO™
Related Products
Description
- Provide topoisomerase-I activated pcDNA3.1/CT-GFP-TOPO vector Taq -amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells Expression is easily detected in living cells using fluorescence Addition, recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g
- GFP) Assays, Fluorescent Protein Assays (GFP), Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: GFP (Cycle 3)
Content And Storage: CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Cloning Method: TOPO-TA
Vector: TOPO-TA Vectors
For Use With (Application): Reporter Assays
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: GFP (Cycle 3)
Quantity: 20 reactions
Product Line: TOPO™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
GFP (Cycle 3)
Content And Storage:
CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Cloning Method:
TOPO-TA
Vector:
TOPO-TA Vectors
For Use With (Application):
Reporter Assays
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
GFP (Cycle 3)
Quantity:
20 reactions
Product Line:
TOPO™
Constitutive or Inducible System: __
Promoter: None (Promoterless)
Reporter Gene: GFP (Cycle 3)
Content And Storage: pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pGlow, TOPO-TA Vectors
For Use With (Application): Reporter Assays, Clone your own Promoter
Delivery Type: Transfection
Product Type: TOPO TA Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: Untagged
Quantity: 20 reactions
Product Line: TOPO™
Constitutive or Inducible System:
__
Promoter:
None (Promoterless)
Reporter Gene:
GFP (Cycle 3)
Content And Storage:
pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pGlow, TOPO-TA Vectors
For Use With (Application):
Reporter Assays, Clone your own Promoter
Delivery Type:
Transfection
Product Type:
TOPO TA Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
Untagged
Quantity:
20 reactions
Product Line:
TOPO™
Constitutive or Inducible System: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
For Use With (Application):
__
Delivery Type:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Protein Tag:
__
Quantity:
__
Product Line:
__
Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: __
Content And Storage: Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method: Directional TOPO
Vector: Directional TOPO Vectors, pcDNA
For Use With (Application): Constitutive Expression
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 Reactions
Product Line: TOPO™, pcDNA™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
__
Content And Storage:
Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method:
Directional TOPO
Vector:
Directional TOPO Vectors, pcDNA
For Use With (Application):
Constitutive Expression
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
20 Reactions
Product Line:
TOPO™, pcDNA™