Invitrogen™ pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | V47020-Each-of-1 | In Stock | ₹ 96,169.44 |
V47020 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 96,169.44
GST (18%): ₹ 17,310.499
Total Price: ₹ 1,13,479.939
Format
Kit
Product Type
β-Galactosidase Reporter Gene Assay System
Content And Storage
The pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit contains 6 μg of vector and 10 μg of pSCREEN-iT™/lacZ-GW/CDK2 control vector, the positive control Stealth™RNAilacZ, a negative control Scrambled Stealth RNAi™ siRNA, and 1X annealing/dilution buffer. Store at -20°C. The BLOCK-iT™ RNAi Target Screening Kit contains the pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit, 250 μl of Lipofectamine™ 2000 Reagent, and the FluoReporter™lacZ/Galactosidase Quantification Kit. Store the Lipofectamine™ 2000 Reagent at +4°C. Store the FluoReporter™ Kit at -20°C protected from light. The BLOCK-iT™ RNAi Target Screening System contains the BLOCK-iT™ RNAi Target Screening Kit, LR Clonase™ enzyme mix, and pCR ¤ 8/GW/TOPO ¤ TA Cloning Kit. Store the LR Clonase™ enzyme mix at -80°C. The pCR™ 8/GW/TOPO™ TA Cloning Kit includes the ready-to-use pCR™ 8/GW/TOPO™ vector, dNTPs, salt solution, water, forward and reverse sequencing primers, control template and PCR primers, One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a pUC19 control plasmid. Store the Competent Cells at -80°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Quantity
20 Reactions
For Use With (Application)
RNAi Target Screening
Promoter
CMV
Reporter Gene
Beta-Gal (lacZ)
Sample Type
Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Product Line
BLOCK-iT™, Gateway™
Related Products
Description
- Use the pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit to assess the potency of any RNAi knockdown reagent using a fast, sensitive, and cost-effective β-gal reporter assay or identify the best knockdown reagent for RNAi experiments without knowing protein function, western blotting, or qRT-PCR
- How it Works: The BLOCK-iT RNAi Target Screening system method uses a β-galactosidase activity assay to measure the knockdown ability of an RNAi reagent (Stealth RNAi™ siRNA, siRNA, dicer pools, or shRNA-containing plasmid)
- To identify the most effective knockdown reagent for your target, simply clone your target gene into the pSCREEN-iT /la cZ-DEST Gateway vector
- Co-transfect the resulting construct with the knockdown reagent being tested
- Expressed genes will be fused to β-galactosidase, enabling you to use β-galactosidase levels to measure the amount of RNAi-induced degradation of the target gene
- The more effective any specific knockdown reagent is at mediating RNAi, the more degradation of the fusion mRNA will occur, lowering β-gal activity
- The BLOCK-iT RNAi Target Screening System includes the pSCREEN-iT / lac Z-DEST Gateway vector for generating a construct containing a la cZ/gene of interest fusion
- This vector features: at tR sites immediately following the la cZ ORF for fast and efficient recombination with any at tL-flanked Gateway entry vector to generate the la cZ/target gene fusions The CMV promoter for strong expression of the la cZ/target gene fusion, leading to increased assay sensitivity and better discrimination between knockdown reagents of varied effectiveness Positional cloning to fuse a gene or fragment to the 3' end of the la cZ gene so that β-galactosidase will be expressed even if the target fragment being tested contains stop codons In addition, the pSCREEN-iT / lac Z-DEST vector is fully compatible with the Ultimate™ ORF Clone Collection
- This makes a wide selection of potential target genes readily available for generating la cZ fusions
- RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Vector-Based RNAi
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Format: Kit
Product Type: β-Galactosidase Reporter Gene Assay System
Content And Storage: The pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit contains 6 μg of vector and 10 μg of pSCREEN-iT™/lacZ-GW/CDK2 control vector, the positive control Stealth™RNAilacZ, a negative control Scrambled Stealth RNAi™ siRNA, and 1X annealing/dilution buffer. Store at -20°C. The BLOCK-iT™ RNAi Target Screening Kit contains the pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit, 250 μl of Lipofectamine™ 2000 Reagent, and the FluoReporter™lacZ/Galactosidase Quantification Kit. Store the Lipofectamine™ 2000 Reagent at +4°C. Store the FluoReporter™ Kit at -20°C protected from light. The BLOCK-iT™ RNAi Target Screening System contains the BLOCK-iT™ RNAi Target Screening Kit, LR Clonase™ enzyme mix, and pCR ¤ 8/GW/TOPO ¤ TA Cloning Kit. Store the LR Clonase™ enzyme mix at -80°C. The pCR™ 8/GW/TOPO™ TA Cloning Kit includes the ready-to-use pCR™ 8/GW/TOPO™ vector, dNTPs, salt solution, water, forward and reverse sequencing primers, control template and PCR primers, One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a pUC19 control plasmid. Store the Competent Cells at -80°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Quantity: 20 Reactions
For Use With (Application): RNAi Target Screening
Promoter: CMV
Reporter Gene: Beta-Gal (lacZ)
Sample Type: Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Product Line: BLOCK-iT™, Gateway™
Format: __
Product Type: Adenovirus Expression Vector
Content And Storage: • pAd⁄PL-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)
Quantity: 6 μg
For Use With (Application): Clone your own Promoter, Viral Expression
Promoter: None (Promoterless)
Reporter Gene: __
Sample Type: __
Product Line: Gateway™, ViraPower™
Format: __
Product Type: Lentiviral Expression Vector
Content And Storage: ViraPower™ Lentiviral Gateway™ Vectors, store at –20°C includes:pLenti6⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mMTris-HCl, 1mM EDTA, pH 8.0)pLenti6⁄V5-GW⁄lacZ control: 10 μg(20 μl of 0.5 μg⁄μl vector in 10 mMTris-HCl, 1mM EDTA, pH 8.0)One Shot™ Stbl3™ Chemically CompetentE. colistore at –80°C
Quantity: 6 μg
For Use With (Application): Viral Expression
Promoter: CMV
Reporter Gene: __
Sample Type: __
Product Line: Gateway™, ViraPower™