K10101
Invitrogen™ Champion™ pET101 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically CompetentE. coli
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K10101-Each-of-1 | In Stock | ₹ 1,02,158.64 |
K10101 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,02,158.64
GST (18%): ₹ 18,388.555
Total Price: ₹ 1,20,547.195
Constitutive or Inducible System
Inducible
Promoter
T7, lacO
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Expression System
E. coli
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
20 reactions
Product Line
One Shot™
Inducing Agent
IPTG
Product Type
TOPO Expression Kit
Content And Storage
Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain
BL21 Star™(DE3)
Expression Mechanism
Cell-Based Expression
Cloning Method
Directional TOPO
Vector
pET
Related Products
Description
- Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because T7 RNA polymerase is more processive than native E
- coli RNA polymerase and is dedicated to transcription of gene of interest Protein production is further enhanced in system by expression strain BL21 Star E
- coli , which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold Simplified, efficient Directional TOPO cloning allows to quickly enter ChampionpET Expression vector Features linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning Directional TOPO Cloning technology facilitates gene expression becausa proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes Greater than 90% of clones are in correct orientation for gene expression, reducing time spent on colony screening Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Inducible
Promoter: T7, lacO
Selection Agent (Eukaryotic): None
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Expression System: E. coli
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 reactions
Product Line: One Shot™
Inducing Agent: IPTG
Product Type: TOPO Expression Kit
Content And Storage: Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: BL21 Star™(DE3)
Expression Mechanism: Cell-Based Expression
Cloning Method: Directional TOPO
Vector: pET
Constitutive or Inducible System:
Inducible
Promoter:
T7, lacO
Selection Agent (Eukaryotic):
None
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Expression System:
E. coli
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
20 reactions
Product Line:
One Shot™
Inducing Agent:
IPTG
Product Type:
TOPO Expression Kit
Content And Storage:
Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain:
BL21 Star™(DE3)
Expression Mechanism:
Cell-Based Expression
Cloning Method:
Directional TOPO
Vector:
pET
Constitutive or Inducible System: Inducible
Promoter: CMV/TO
Selection Agent (Eukaryotic): Zeocin™, Blasticidin
Antibiotic Resistance Bacterial: __
Expression System: __
Protein Tag: Untagged
Quantity: 1 kit
Product Line: T-REx™, pcDNA™
Inducing Agent: Tetracycline
Product Type: T-Rex Complete Cloning Kit with Vector
Content And Storage: The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO, a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.The T-REx™ Complete Kit includes the Core Kit plus 1 g Zeocin™ (100 mg/ml), 50 mg of Blasticidin, and 5 g of tetracycline.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. Zeocin™ and tetracycline should be protected from exposure to light. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: __
Expression Mechanism: __
Cloning Method: Restriction Enzyme/MCS
Vector: pcDNA
Constitutive or Inducible System:
Inducible
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Zeocin™, Blasticidin
Antibiotic Resistance Bacterial:
__
Expression System:
__
Protein Tag:
Untagged
Quantity:
1 kit
Product Line:
T-REx™, pcDNA™
Inducing Agent:
Tetracycline
Product Type:
T-Rex Complete Cloning Kit with Vector
Content And Storage:
The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO, a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.The T-REx™ Complete Kit includes the Core Kit plus 1 g Zeocin™ (100 mg/ml), 50 mg of Blasticidin, and 5 g of tetracycline.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. Zeocin™ and tetracycline should be protected from exposure to light. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain:
__
Expression Mechanism:
__
Cloning Method:
Restriction Enzyme/MCS
Vector:
pcDNA
Constitutive or Inducible System: Inducible
Promoter: CMV/TO
Selection Agent (Eukaryotic): Zeocin™, Blasticidin
Antibiotic Resistance Bacterial: __
Expression System: __
Protein Tag: Untagged
Quantity: 1 kit
Product Line: Gateway™, T-REx™, pcDNA™
Inducing Agent: Tetracycline
Product Type: T-Rex Core Cloning Kit with Vector
Content And Storage: The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO or 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain: __
Expression Mechanism: __
Cloning Method: Restriction Enzyme/MCS
Vector: pcDNA
Constitutive or Inducible System:
Inducible
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Zeocin™, Blasticidin
Antibiotic Resistance Bacterial:
__
Expression System:
__
Protein Tag:
Untagged
Quantity:
1 kit
Product Line:
Gateway™, T-REx™, pcDNA™
Inducing Agent:
Tetracycline
Product Type:
T-Rex Core Cloning Kit with Vector
Content And Storage:
The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO or 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain:
__
Expression Mechanism:
__
Cloning Method:
Restriction Enzyme/MCS
Vector:
pcDNA
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Expression System: __
Protein Tag: __
Quantity: 5 mg
Product Line: GeneBLAzer™
Inducing Agent: __
Product Type: __
Content And Storage: Store in freezer (-5 to -30°C).
Bacterial or Yeast Strain: __
Expression Mechanism: __
Cloning Method: __
Vector: __
Constitutive or Inducible System:
__
Promoter:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
__
Expression System:
__
Protein Tag:
__
Quantity:
5 mg
Product Line:
GeneBLAzer™
Inducing Agent:
__
Product Type:
__
Content And Storage:
Store in freezer (-5 to -30°C).
Bacterial or Yeast Strain:
__
Expression Mechanism:
__
Cloning Method:
__
Vector:
__