Invitrogen™ Champion™ pET SUMO Expression System
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K30001-Each-of-1 | In Stock | ₹ 1,26,799.92 |
K30001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,26,799.92
GST (18%): ₹ 22,823.986
Total Price: ₹ 1,49,623.906
Constitutive or Inducible System
Inducible
Promoter
T7, lacO
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Kanamycin (KanR)
Cleavage
SUMO
Expression Mechanism
Cell-Based Expression
Cloning Method
TOPO
Vector
pET
Inducing Agent
IPTG
Product Type
Expression System
Content And Storage
The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning™ box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot™ TOP10 box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C.
Bacterial or Yeast Strain
BL21(DE3)
Expression System
E. coli
Protein Tag
His Tag (6x), SUMO Tag
Quantity
20 reactions
Product Line
Champion™, TA Cloning™
Related Products
Description
- Uses small ubiquitin-related modifier (SUMO) fusion, belonging to growing family of ubiquitin-related proteins, to enhance solubility of expressed fusion proteins Contrast to ubiquitin, SUMO is involved in stabilization and localization of proteins in vivo After expression, 11 kd SUMO moiety can be cleaved by highly specific and active SUMO (ULP-1) protease at carboxyl terminal, producing native protein Enhanced solubility with N-terminal SUMO fusion Highly efficient cleavage produces native protein of interest with SUMO (ULP-1) protease Highly specific cleavage eliminates chance of protein of interest being internally digested, regardless of its amino acid sequence Significantly increased stability with SUMO fusion-can be used for small peptide productionT 7 lac promoter for high-level protein expression N-terminal 6xHis tag for protein detection and purification Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Inducible
Promoter: T7, lacO
Selection Agent (Eukaryotic): None
Antibiotic Resistance Bacterial: Kanamycin (KanR)
Cleavage: SUMO
Expression Mechanism: Cell-Based Expression
Cloning Method: TOPO
Vector: pET
Inducing Agent: IPTG
Product Type: Expression System
Content And Storage: The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning™ box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot™ TOP10 box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C.
Bacterial or Yeast Strain: BL21(DE3)
Expression System: E. coli
Protein Tag: His Tag (6x), SUMO Tag
Quantity: 20 reactions
Product Line: Champion™, TA Cloning™
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Vector: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Cloning Method: __
Vector: __
Inducing Agent: __
Product Type: __
Content And Storage: __
Bacterial or Yeast Strain: __
Expression System: __
Protein Tag: __
Quantity: __
Product Line: __
