Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant

Description

• Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA
• Source: Purified from baculovirus clone of calf thymus TdT Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr
• at 37°C, using d(pA) 50 as primer Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO 4 C 4 H 6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA) 50 , 1mM [ 3 H]dATP, and enzyme in 0.15mL for 1 h at 37°C Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32 P- or 35 S-label; Apoptosis (TUNEL) Order Info Shipping Conditions: Approved for shipment on wet or dry ice