15-224-017

Invitrogen™ T4 DNA Ligase (1 U/μL)

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 15-224-017-Each-of-1 In Stock ₹ 8,898.24

15-224-017 - Each of 1

₹ 8,898.24

In Stock

Quantity

1

Base Price: ₹ 8,898.24

GST (18%): ₹ 1,601.683

Total Price: ₹ 10,499.923

Concentration

1 U/μL

Shipping Condition

Dry Ice

Compatible Buffer

DNA Ligase Buffer, 5X Reaction Buffer

Product Type

T4 DNA Ligase

Content And Storage

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme

Ligase

Quantity

100 U

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Description

  • T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini
  • Single-stranded nucleic acids are not substrates for this enzyme
  • Optimizes ligation in 5 minutes Source: Purified from E
  • coli lambda lysogen NM989 Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested Unit definition: One unit catalyzes exchange of 1nmol 32 P-labeled pyrophosphate into ATP in 20 min
  • at 37°C
  • One unit is equal to approximately 300 cohesive-end ligation units Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl 2 , 10mM DTT, 66μM ATP, 3.3μM 32 P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min
  • at 37°C ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning Order Info Shipping Conditions: Dry ice

Compare Similar Items

Show Difference

Img

Fischer Scientific

15-224-017

--


Concentration:
1 U/μL

Shipping Condition:
Dry Ice

Compatible Buffer:
DNA Ligase Buffer, 5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
100 U

Img

Fischer Scientific

15-224-025

--


Concentration:
1 U/μL

Shipping Condition:
Dry Ice

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
500 U

Img

Invitrogen™

15-224-041

--


Concentration:
5 U/μL

Shipping Condition:
Dry Ice

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
250 U

Img

Fischer Scientific

15-224-090

--


Concentration:
1 U/μL

Shipping Condition:
__

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
4 x 500 U