15-224-025

Invitrogen™ T4 DNA Ligase (1 U/μL)

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 15-224-025-Each-of-1 In Stock ₹ 29,347.08

15-224-025 - Each of 1

₹ 29,347.08

In Stock

Quantity

1

Base Price: ₹ 29,347.08

GST (18%): ₹ 5,282.474

Total Price: ₹ 34,629.554

Concentration

1 U/μL

Shipping Condition

Dry Ice

Compatible Buffer

5X Reaction Buffer

Product Type

T4 DNA Ligase

Content And Storage

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme

Ligase

Quantity

500 U

Related Products

Img

Invitrogen™

15-224-041

--

Img

Fischer Scientific

15-224-090

--

Img

Fischer Scientific

15-224-017

--

Img

Invitrogen™

46-300-018

--

Img

Invitrogen™

18-052-019

--

Img

Invitrogen™

18-001-016

--

Img

Invitrogen™

18-011-015

--

Img

Fischer Scientific

10-533-065

--

Description

  • T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini
  • Single-stranded nucleic acids are not substrates for this enzyme
  • Optimizes ligation in 5 minutes Source: Purified from E
  • coli lambda lysogen NM989 Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested Unit definition: One unit catalyzes exchange of 1nmol 32 P-labeled pyrophosphate into ATP in 20 min
  • at 37°C
  • One unit is equal to approximately 300 cohesive-end ligation units Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl 2 , 10mM DTT, 66μM ATP, 3.3μM 32 P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min
  • at 37°C ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning Order Info Shipping Conditions: Dry ice

Compare Similar Items

Show Difference

Img

Fischer Scientific

15-224-025

--


Concentration:
1 U/μL

Shipping Condition:
Dry Ice

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
500 U

Img

Invitrogen™

15-224-041

--


Concentration:
5 U/μL

Shipping Condition:
Dry Ice

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
250 U

Img

Fischer Scientific

15-224-090

--


Concentration:
1 U/μL

Shipping Condition:
__

Compatible Buffer:
5X Reaction Buffer

Product Type:
T4 DNA Ligase

Content And Storage:
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme:
Ligase

Quantity:
4 x 500 U

Img

Fischer Scientific

15-226-16

--


Concentration:
__

Shipping Condition:
__

Compatible Buffer:
__

Product Type:
Slip-joint Pliers

Content And Storage:
__

Enzyme:
__

Quantity:
__