18-052-019
Invitrogen™E. coliDNA Ligase
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-052-019-Each-of-1 | In Stock | ₹ 5,775.30 |
18-052-019 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,775.30
GST (18%): ₹ 1,039.554
Total Price: ₹ 6,814.854
Content And Storage
E. coliDNA Ligase is supplied with a vial of 10X reaction buffer [188 mM Tris-HCl (pH 8.3), 906 mM KCl, 46 mM MgCl2, 37.5 mMDTT, 1.5 mM λ-NAD, 100 mM (NH4)2SO4]. Store at -20°C.
Enzyme
Ligase
Quantity
100 U
Shipping Condition
Approved for shipment on Wet or Dry Ice
Compatible Buffer
10X Reaction Buffer
Product Type
DNA Ligase
Related Products
Description
- Single-stranded nucleic acids are not substrates for this enzyme
- Source: Purified from E
- coli 594 ( Su - ) bearing λ lysogen gt4lop-11 lig + S7 Performance and Quality Testing: Endodeoxyribonuclease and 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested Unit Definition: One unit is amount of enzyme required to give 50% ligation of Hin d III-digested λ DNA in 30 min
- at 16°C in a final volume of 20μL containing a 5' termini concentration of 0.12 'M (300μg/mL) Unit Reaction Conditions: 18.8mM Tris-HCl (pH 8.3), 90.6mM KCl, 4.6mM MgCl 2 , 3.8mM DTT, 0.15mM λ-NAD, 10mM (NH 4 ) 2 SO 4 in 20μL for 1 hr
- at 16°C Second-strand cDNA synthesis; T4 DNA Ligase alternative when blunt-end ligation is not required; Cloning, Restriction Enzyme Cloning Order Info Shipping Conditions: Approved for shipment on wet or dry ice
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Content And Storage: E. coliDNA Ligase is supplied with a vial of 10X reaction buffer [188 mM Tris-HCl (pH 8.3), 906 mM KCl, 46 mM MgCl2, 37.5 mMDTT, 1.5 mM λ-NAD, 100 mM (NH4)2SO4]. Store at -20°C.
Enzyme: Ligase
Quantity: 100 U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Compatible Buffer: 10X Reaction Buffer
Product Type: DNA Ligase
Content And Storage:
E. coliDNA Ligase is supplied with a vial of 10X reaction buffer [188 mM Tris-HCl (pH 8.3), 906 mM KCl, 46 mM MgCl2, 37.5 mMDTT, 1.5 mM λ-NAD, 100 mM (NH4)2SO4]. Store at -20°C.
Enzyme:
Ligase
Quantity:
100 U
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Compatible Buffer:
10X Reaction Buffer
Product Type:
DNA Ligase
Content And Storage: Store in freezer (-5° to -30°C).
Enzyme: __
Quantity: 100 units
Shipping Condition: Approved for shipment on Wet or Dry Ice
Compatible Buffer: __
Product Type: __
Content And Storage:
Store in freezer (-5° to -30°C).
Enzyme:
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Quantity:
100 units
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Compatible Buffer:
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Product Type:
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Content And Storage: __
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Shipping Condition: __
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