3509037
Promega Terminal Deoxynucleotidyl Transferase
TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Concentration
30 U/μL
Components
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
For Use With (Application)
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage
-30°C to -10°C
Product Type
Terminal Deoxynucleotidyl Transferase
Color
Colorless
pH
6.4
Storage Buffer
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source
Recombinant E. coli strain
Form
Liquid
Related Products
Description
- Adds Mononucleotides to the Terminal 3′-OH of ssDNA and Releases PPi Adds mononucleotide tails to any type of 3′ end (3′ and 5′ overhangs or blunt ends) due to the presence of 1mM CoCl2 in the reaction buffer Quality tested for labeling of apoptotic DNA ends using the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235 for each lot of enzyme Supplied at a concentration of 30u/μL
Compare Similar Items
Show Difference
Concentration: 30 U/μL
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Product Type: Terminal Deoxynucleotidyl Transferase
Color: Colorless
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Form: Liquid
Concentration:
30 U/μL
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Product Type:
Terminal Deoxynucleotidyl Transferase
Color:
Colorless
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Form:
Liquid
Concentration: 150 to 200 U/μL
Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage: -30°C to -10°C
Product Type: Exonuclease III
Color: Colorless
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source: Recombinant E. coli strain
Form: Liquid
Concentration:
150 to 200 U/μL
Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage:
-30°C to -10°C
Product Type:
Exonuclease III
Color:
Colorless
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source:
Recombinant E. coli strain
Form:
Liquid
Concentration: __
Components: __
For Use With (Application): __
Content And Storage: The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Type: __
Color: __
pH: __
Storage Buffer: __
Source: __
Form: __
Concentration:
__
Components:
__
For Use With (Application):
__
Content And Storage:
The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Type:
__
Color:
__
pH:
__
Storage Buffer:
__
Source:
__
Form:
__
Concentration: __
Components: __
For Use With (Application): __
Content And Storage: __
Product Type: __
Color: Green
pH: __
Storage Buffer: __
Source: __
Form: __
Concentration:
__
Components:
__
For Use With (Application):
__
Content And Storage:
__
Product Type:
__
Color:
Green
pH:
__
Storage Buffer:
__
Source:
__
Form:
__