Invitrogen™ AcTEV™ Protease

Description

• An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity
• Highly specific cleavage activity Enhanced enzyme stability for prolonged protease activity Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range Six-histidine sequence to facilitate its removal from the digested protein sample Greater than 85% single-band purity with no nonspecific protease contamination Application Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein Enzyme specifications Purified from E
• coli expressing the AcTEV™ Protease gene Unit definition One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C Unit reaction conditions 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice