Fisher BioReagents™ Optizyme™ Ribonuclease Inhibitor, Recombinant

Description

• Supplied as a recombinant product from E
• coli (originally isolated from rat lung) and provides superior protection of RNA from degradation by RNases Commonly added to all solutions used during the isolation of RNA, and will not interfere with the performance of most enzymes in downstream applications Active over a broad temperature range, and even provides some RNase inhibition at 60°C, which is useful when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA Storage Buffer Components: 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, and 50% glycerol (v/v) Inactivates RNase A, RNase B, and RNase T2; No activity against SP6, T7, or T3 RNA polymerases, AMV or MMLV reverse transcriptases, and Taq DNA polymerase Displays a broad spectrum of inhibitory activity against RNases, and does not have activity against other polymerases and reverse transcriptases Suitable for use in common molecular biology applications such as isolation and purification of RNA, cDNA synthesis, RT-PCR, in vitro RNA transcription/translation, ribonuclease protection assay, and preparation of RNase-free antibodies