3509013
Promega RNase One™
Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Concentration
5 to 10 U/μL
Components
10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
For Use With (Application)
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage
-30°C to -10°C
Product Type
Ribonuclease
Form
Liquid
Color
Colorless
pH
7.3
Storage Buffer
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source
Recombinant E. coli strain
Molecular Weight (g/mol)
27
Related Products
Description
- Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates Ability to cleave a phosphodiester bond between any two ribonucleotides Supplied at a concentration of 5-10u/μL
Compare Similar Items
Show Difference
Concentration: 5 to 10 U/μL
Components: 10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
For Use With (Application): Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage: -30°C to -10°C
Product Type: Ribonuclease
Form: Liquid
Color: Colorless
pH: 7.3
Storage Buffer: 10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 27
Concentration:
5 to 10 U/μL
Components:
10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
For Use With (Application):
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage:
-30°C to -10°C
Product Type:
Ribonuclease
Form:
Liquid
Color:
Colorless
pH:
7.3
Storage Buffer:
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
27
Concentration: __
Components: 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Product Type: SP6 RNA Polymerase
Form: Liquid
Color: Colorless
pH: 7.5
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): __
Concentration:
__
Components:
100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Product Type:
SP6 RNA Polymerase
Form:
Liquid
Color:
Colorless
pH:
7.5
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
__
Concentration: __
Components: 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
For Use With (Application): Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage: -30°C to -10°C
Product Type: T4 DNA Ligase
Form: Liquid
Color: Colorless
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: RecombinantE. colistrain
Molecular Weight (g/mol): 68
Concentration:
__
Components:
10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
For Use With (Application):
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage:
-30°C to -10°C
Product Type:
T4 DNA Ligase
Form:
Liquid
Color:
Colorless
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
RecombinantE. colistrain
Molecular Weight (g/mol):
68
Concentration: 5 to 10 U/μL
Components: 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
For Use With (Application): 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage: -30°C to -10°C
Product Type: T4 Polynucleotide Kinase
Form: Liquid
Color: Colorless
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 132
Concentration:
5 to 10 U/μL
Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage:
-30°C to -10°C
Product Type:
T4 Polynucleotide Kinase
Form:
Liquid
Color:
Colorless
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
132