3509011
Promega Ribonuclease H
RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.
Manufacturer: Fischer Scientific
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Concentration
0.5 to 2 U/μL
pH
7.8
Storage Buffer
20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Source
Recombinant E. coli strain
Form
Liquid
Color
Colorless
For Use With (Application)
Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Content And Storage
-30°C to -10°C
Product Type
Ribonuclease H
Related Products
Description
- Endonuclease that Specifically Hydrolyzes the Phosphodiester Bonds of RNA Hybridized to DNA Removes the RNA strand prior to second-strand cDNA synthesis Analyzes in vitro polyadenylation reaction products Supplied at a concentration of 0.5-2u/μL
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Concentration: 0.5 to 2 U/μL
pH: 7.8
Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Source: Recombinant E. coli strain
Form: Liquid
Color: Colorless
For Use With (Application): Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Content And Storage: -30°C to -10°C
Product Type: Ribonuclease H
Concentration:
0.5 to 2 U/μL
pH:
7.8
Storage Buffer:
20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Source:
Recombinant E. coli strain
Form:
Liquid
Color:
Colorless
For Use With (Application):
Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Content And Storage:
-30°C to -10°C
Product Type:
Ribonuclease H
Concentration: 5 to 10 U/μL
pH: 7.3
Storage Buffer: 10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source: Recombinant E. coli strain
Form: Liquid
Color: Colorless
For Use With (Application): Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage: -30°C to -10°C
Product Type: Ribonuclease
Concentration:
5 to 10 U/μL
pH:
7.3
Storage Buffer:
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source:
Recombinant E. coli strain
Form:
Liquid
Color:
Colorless
For Use With (Application):
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage:
-30°C to -10°C
Product Type:
Ribonuclease
Concentration: __
pH: 7.5
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Form: Liquid
Color: Colorless
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Product Type: SP6 RNA Polymerase
Concentration:
__
pH:
7.5
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Form:
Liquid
Color:
Colorless
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Product Type:
SP6 RNA Polymerase
Concentration: __
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: RecombinantE. colistrain
Form: Liquid
Color: Colorless
For Use With (Application): Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage: -30°C to -10°C
Product Type: T4 DNA Ligase
Concentration:
__
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
RecombinantE. colistrain
Form:
Liquid
Color:
Colorless
For Use With (Application):
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage:
-30°C to -10°C
Product Type:
T4 DNA Ligase