OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

Description

• OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA
• Applications and Features: OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments Fast sticky end ligation (10min at room temperature) Cloning PCR products Ligation of double-stranded oligonucleotide linkers or adaptors to DNA Site-directed mutagenesis Amplified fragment length polymorphism Ligase-mediated RNA detection Nick repair in duplex DNA, RNA or DNA/RNA hybrids Active in buffers for OPTIZYME restriction enzymes Supplied with: 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl 2 , 100mM DTT, 5mM ATP (pH7.8 at 25°C) 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol Source: E
• Coli cells with a cloned gene 30 of bacteriophage T4 Quality control: Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free Functionally tested for ligation of cohesive and blunt end DNA fragments