BP81051
OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
5 U/μL
pH
7.5
Storage Buffer
OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Source
E. coli cells with a cloned gene 43 of bacteriophage T4
Product Type
T4 DNA Polymerase
Color
Colorless
For Use With (Application)
Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Content And Storage
-20°C
Quantity
100 U
Form
Liquid
Related Products
Description
- OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA
- Applications and features: OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs Creation of blunt PCR products with 3'-dA overhangs Oligonucleotide-directed site-specific mutagenesis Synthesis of labeled DNA probes (by replacement reaction) Ligation-independent cloning of PCR products Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase 3'->5' exonuclease activity Lacks 5'->3' exonuclease activity Supplied with: 5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl 2 , 5mM DTT, 84mM (NH 4 ) 2 SO 4 Source: E
- Coli cells with a cloned gene 43 of bacteriophage T4 Quality Control: Endodeoxyribonuclease-free
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Concentration: 5 U/μL
pH: 7.5
Storage Buffer: OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Source: E. coli cells with a cloned gene 43 of bacteriophage T4
Product Type: T4 DNA Polymerase
Color: Colorless
For Use With (Application): Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Content And Storage: -20°C
Quantity: 100 U
Form: Liquid
Concentration:
5 U/μL
pH:
7.5
Storage Buffer:
OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Source:
E. coli cells with a cloned gene 43 of bacteriophage T4
Product Type:
T4 DNA Polymerase
Color:
Colorless
For Use With (Application):
Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Content And Storage:
-20°C
Quantity:
100 U
Form:
Liquid
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