Gibco™ Human MRP4 Vesicles

Description

• Human MRP4 Vesicles are insect derived purified plasma membranes with inserted MRP4 transport protein (multidrug resistance-associated protein 4, ABCC4)
• Our Human MRP4 Vesicles can be used to evaluate test compounds and drug interactions with MRP4 in in vitro assays
• MRP4 is an ATP-binding cassette (ABC) transporter that carries organic anions, including nucleic acid derivatives, prostaglandins, and methotrexate
• Use Human MRP4 Vesicles to: Investigate the transporter interactions of your drug candidates Assess potential for transporter-mediated drug-drug interactions Obtain high quality results with a large signal to noise ratios Concept Behind ABC Transporter Vesicles ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential
• While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles
• It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles
• Clear and Reliable Results Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter
• Transporter Vesicle Details Human MRP4 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles
• They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP4
• MRP4 is highly expressed in proximal uriniferous tubules in the kidney, and plays an important role in drug excretion from the kidney
• MRP4 has also been identified in vascular endothelial cells of the brain, where it greatly influences drug behavior at the blood-brain barrier
• For Research Use Only
• Not intended for animal or human therapeutic or diagnostic use.