Cut at ^GATC sites with Bsp143I (Sau3AI), which performs best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).
Manufacturer: Fischer Scientific
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10 U/μL
Bsp143I (Sau3AI)
Yes
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Unique Buffer (10X Buffer Bsp143I (Sau3AI))
37°C
Restriction Enzyme