4521762

Thermo Scientific™ Eco57I (AcuI) (5 U/μL)

The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI).

Manufacturer: Fischer Scientific

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Concentration

5 U/μL

Enzyme

Eco57I (AcuI)

Sensitive to Heat Inactivation

Yes

Type IIS RE

Yes

Research Category

Scarless Cloning

Methylation Sensitivity

Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive

Compatible Buffer

10X Buffer G

Optimal Reaction Temperature

37°C

Product Type

Restriction Enzyme

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Description

  • Lambda DNA digested with Eco57I (AcuI), 1% agarose, 40 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
  • In addition, the universal Tango buffer is provided for convenience in double digestions
  • All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
  • To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
  • Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities

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Fischer Scientific

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The Eco57I (AcuI) restriction enzym...


Concentration:
5 U/μL

Enzyme:
Eco57I (AcuI)

Sensitive to Heat Inactivation:
Yes

Type IIS RE:
Yes

Research Category:
Scarless Cloning

Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive

Compatible Buffer:
10X Buffer G

Optimal Reaction Temperature:
37°C

Product Type:
Restriction Enzyme

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Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive

Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer

Optimal Reaction Temperature:
37°C

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