The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI).
Manufacturer: Fischer Scientific
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5 U/μL
Eco57I (AcuI)
Yes
Yes
Scarless Cloning
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer G
37°C
Restriction Enzyme