4521889
Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
Manufacturer: Fischer Scientific
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Concentration
5 U/μL
Enzyme
LguI (SapI)
Sensitive to Heat Inactivation
Yes
Type IIS RE
Yes
Research Category
Scarless Cloning
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X Buffer Tango
Optimal Reaction Temperature
37°C
Product Type
Restriction Enzyme
Related Products
Description
- Lambda DNA digested with LguI (SapI), 0.7% agarose, 10 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities
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Concentration: 5 U/μL
Enzyme: LguI (SapI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Research Category: Scarless Cloning
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
5 U/μL
Enzyme:
LguI (SapI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Research Category:
Scarless Cloning
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: __
Enzyme: __
Sensitive to Heat Inactivation: __
Type IIS RE: __
Research Category: __
Methylation Sensitivity: __
Compatible Buffer: __
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Product Type: Sample Container
Concentration:
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Enzyme:
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Sensitive to Heat Inactivation:
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Type IIS RE:
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Research Category:
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Methylation Sensitivity:
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Compatible Buffer:
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Product Type:
Sample Container
Concentration: __
Enzyme: __
Sensitive to Heat Inactivation: __
Type IIS RE: __
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Concentration:
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Enzyme:
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Sensitive to Heat Inactivation:
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Type IIS RE:
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Research Category:
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Methylation Sensitivity:
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Compatible Buffer:
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Optimal Reaction Temperature:
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Product Type:
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Concentration: 10 U/μL
Enzyme: __
Sensitive to Heat Inactivation: __
Type IIS RE: __
Research Category: __
Methylation Sensitivity: __
Compatible Buffer: __
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Concentration:
10 U/μL
Enzyme:
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Sensitive to Heat Inactivation:
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Type IIS RE:
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Research Category:
__
Methylation Sensitivity:
__
Compatible Buffer:
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Optimal Reaction Temperature:
__
Product Type:
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