Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).
Manufacturer: Fischer Scientific
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10 U/μL
BseGI (BtsCI)
Yes
Yes
Scarless Cloning
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer Tango
55°C
Restriction Enzyme