4521872
Thermo Scientific™ LweI (SfaNI) (10 U/μL)
The LweI (SfaNI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: SfaNI).
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Enzyme
LweI (SfaNI)
Sensitive to Heat Inactivation
Yes
Type IIS RE
Yes
Research Category
Scarless Cloning
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X Buffer Tango
Optimal Reaction Temperature
37°C
Product Type
Restriction Enzyme
Related Products
Description
- Lambda DNA digested with LweI (SfaNI), 1.4% agarose, 169 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities
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Concentration: 10 U/μL
Enzyme: LweI (SfaNI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Research Category: Scarless Cloning
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
10 U/μL
Enzyme:
LweI (SfaNI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Research Category:
Scarless Cloning
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: 5 U/μL
Enzyme: LguI (SapI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Research Category: Scarless Cloning
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
5 U/μL
Enzyme:
LguI (SapI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Research Category:
Scarless Cloning
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: __
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Sensitive to Heat Inactivation: __
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Methylation Sensitivity:
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