Cut at CCANNNN^NTGG sites with Van91I (PflMI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccB7I, BasI, PflMI).
Manufacturer: Fischer Scientific
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10 U/μL
Van91I (PflMI)
Yes
No
Traditional Cloning
Dcm Methylation-Sensitive, Not Dam Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer R
37°C
Restriction Enzyme