Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Description

• Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels
• You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer
• Usage Recommendations Use fresh 1X TAE both for the gel and for the electrophoresis run To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well Quality Control The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests 1X Composition 40mM Tris 20mM acetic acid 1mM EDTA pHof 50X TAE: 8.4 Recommended for: Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l
• Note: TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.