Thermo Scientific™ Tango Buffer (10X)

Description

• Our Five Buffer System ensures the optimum reaction conditions for each restriction enzyme
• This system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers
• All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer
• The recommended buffer and/or the universal Tango buffer are supplied with each enzyme
• To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
• Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation
• Restriction enzymes exhibit 100% of their certified activity in the recommended buffer
• However, some enzymes require additives to achieve 100% activity
• For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme)
• Esp3I requires DTT
• Tango buffer has been designed for double digestions of DNA with conventional restriction enzymes.