Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO

Description

• A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between the two groups This crosslinker can be used to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately) The coupling or crosslinking procedure is typically done in two steps: First, a protein or amine containing molecule is labeled with a DBCO group and excess crosslinker is removed Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures TFP esters are more hydrolytically stable than NHS esters