Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO

Description

• EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach
• The DBCO group reacts preferentially with an azide through a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form a stable triazole linkage with an azide-labeled coupling partner
• EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and reach
• EZ-Link Biotin-PEG12-DBCO features include: • Bioorthogonal—DBCO and azides react selectively to form triazole linkages • Enhanced solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution • Irreversible—forms irreversible and stable triazole linkage Classic click reactions involve a copper-catalyzed azide-alkyne cyclo­addition to label or conjugate molecules
• A drawback of this approach is that copper ions can impair protein function, harm cells, and reduce the fluorescence of fluorophores
• DBCO is distinct in that it doesn’t require copper to catalyze the reaction with an azide for triazole formation
• The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, preventing copper-induced damage to cells or proteins.