Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO

Description

• A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately) The coupling or crosslinking procedure is typically done in two steps: First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures