FERER0221
Thermo Scientific™ DraI (10 U/μL)
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERER0221-Each-of-1 | In Stock | ₹ 6,648.01 |
FERER0221 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 6,648.01
GST (18%): ₹ 1,196.642
Total Price: ₹ 7,844.652
Concentration
10 U/μL
Enzyme
Dra I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X Buffer Tango
Optimal Reaction Temperature
37°C
Quantity
1500 units
Research Category
Traditional Cloning
Related Products
Description
- Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion
- 5'...T T T▵A A A...3' 3'...A A A▵T T T...5' Conditions for 100% Activity: 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA Incubate at 37°C Storage Buffer: DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol Ligation and Recleavage: After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut Methylation Effects: Dam: never overlaps — no effect Dcm: never overlaps — no effect CpG: never overlaps — no effect EcoKI: may overlap — blocked EcoBI: never overlaps — no effect Digestion of Agarose-embedded DNA: Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
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Concentration: 10 U/μL
Enzyme: Dra I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Quantity: 1500 units
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Dra I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Quantity:
1500 units
Research Category:
Traditional Cloning
Concentration: 50 U/μL
Enzyme: Dra I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Quantity: 7500 U
Research Category: Traditional Cloning
Concentration:
50 U/μL
Enzyme:
Dra I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Quantity:
7500 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Eam1104I (EarI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Quantity: 300 U
Research Category: Scarless Cloning
Concentration:
10 U/μL
Enzyme:
Eam1104I (EarI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Quantity:
300 U
Research Category:
Scarless Cloning
Concentration: 10 U/μL
Enzyme: Eam1104I (EarI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Quantity: 1500 U
Research Category: Scarless Cloning
Concentration:
10 U/μL
Enzyme:
Eam1104I (EarI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Quantity:
1500 U
Research Category:
Scarless Cloning