FERER0593
Thermo Scientific™ NotI, HC (50 U/μL)
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERER0593-Each-of-1 | In Stock | ₹ 27,250.86 |
FERER0593 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,250.86
GST (18%): ₹ 4,905.155
Total Price: ₹ 32,156.015
Concentration
50 U/μL
Enzyme
Not I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X Buffer O
Optimal Reaction Temperature
37°C
Quantity
1500 units
Research Category
Traditional Cloning
Related Products
Description
- Thermo Scientific™ NotI is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.5μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with NotI
- 5'..G C▵G G C C G C...3' 3'..C G C C G G▵C G...5' Conditions for 100% Activity: 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl 2 , 100mM NaCl and 0.1mg/mL BSA Incubate at 37°C Storage Buffer: NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol Ligation and Recleavage: After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recut Methylation Effects: Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: completely overlaps —blocked EcoKI: never overlaps —no effect EcoBI: never overlaps —no effect Digestion of Agarose-embedded DNA: Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours Compatible Ends: Bsp120I, CfrI, Eco52I Note: Assayed using pTZ19RJL2 plasmid DNA digested with BseLI
- Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.
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Concentration: 50 U/μL
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer O
Optimal Reaction Temperature: 37°C
Quantity: 1500 units
Research Category: Traditional Cloning
Concentration:
50 U/μL
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer O
Optimal Reaction Temperature:
37°C
Quantity:
1500 units
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer O
Optimal Reaction Temperature: 37°C
Quantity: 500 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer O
Optimal Reaction Temperature:
37°C
Quantity:
500 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Pst I
Sensitive to Heat Inactivation: No
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer O
Optimal Reaction Temperature: 37°C
Quantity: __
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Pst I
Sensitive to Heat Inactivation:
No
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer O
Optimal Reaction Temperature:
37°C
Quantity:
__
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Pst I
Sensitive to Heat Inactivation: No
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer O
Optimal Reaction Temperature: 37°C
Quantity: 5 x 3000 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Pst I
Sensitive to Heat Inactivation:
No
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer O
Optimal Reaction Temperature:
37°C
Quantity:
5 x 3000 U
Research Category:
Traditional Cloning