PI75705

Thermo Scientific™ beta-Galactosidase Assay Reagent

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PI75705-Each-of-1 In Stock ₹ 15,452.14

PI75705 - Each of 1

₹ 15,452.14

In Stock

Quantity

1

Base Price: ₹ 15,452.14

GST (18%): ₹ 2,781.385

Total Price: ₹ 18,233.525

Assay

Reporter Enzyme

Detection Method

Colorimetric

For Use With (Application)

Absorbance

Quantity

25 mL

Substrate Properties

Chemical Substrate

Format

96-well Plate

Label or Dye

Enzyme Labeled

Content And Storage

Store at -20°C.

Form

Liquid

Product Line

Pierce™

Substrate

Beta-Gal Substrate

Target Enzyme

Beta-Galactosidase

For Use With (Equipment)

Microplate Reader

Product Type

Pierce beta-Galactosidase Assay Reagent

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Description

  • Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit
  • The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity
  • Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution
  • Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity
  • With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles
  • The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification
  • Highlights: Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatiible with prepared lysates from other species such as bacteria Includes: Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No
  • 78503), and ready-to-use Stop Solution

Compare Similar Items

Show Difference

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Fischer Scientific

PI75705

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Assay:
Reporter Enzyme

Detection Method:
Colorimetric

For Use With (Application):
Absorbance

Quantity:
25 mL

Substrate Properties:
Chemical Substrate

Format:
96-well Plate

Label or Dye:
Enzyme Labeled

Content And Storage:
Store at -20°C.

Form:
Liquid

Product Line:
Pierce™

Substrate:
Beta-Gal Substrate

Target Enzyme:
Beta-Galactosidase

For Use With (Equipment):
Microplate Reader

Product Type:
Pierce beta-Galactosidase Assay Reagent

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Assay:
__

Detection Method:
Reporter Enzyme

For Use With (Application):
Absorbance

Quantity:
75 mL Kit

Substrate Properties:
__

Format:
96-well Plate

Label or Dye:
__

Content And Storage:
Sufficient For: 250 microplate wells of adherent cellsPierce beta-Galactosidase Assay Reagent, 25 mLM-PER Mammalian Protein Extraction Reagent, 25 mLStop Solution, 25 mLStore the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.

Form:
__

Product Line:
Pierce™

Substrate:
Proprietary

Target Enzyme:
__

For Use With (Equipment):
Microplate Reader

Product Type:
Beta-Galactosidase Assay Kit

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Assay:
Reporter Enzyme

Detection Method:
Colorimetric

For Use With (Application):
Absorbance

Quantity:
3 x 25 mL

Substrate Properties:
Chemical Substrate

Format:
96-well Plate

Label or Dye:
Enzyme Labeled

Content And Storage:
Store at -20°C.

Form:
Liquid

Product Line:
Pierce™

Substrate:
Beta-Gal Substrate

Target Enzyme:
Beta-Galactosidase

For Use With (Equipment):
Microplate Reader

Product Type:
Pierce beta-Galactosidase Assay Reagent

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Assay:
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Detection Method:
Reporter Enzyme

For Use With (Application):
Absorbance

Quantity:
75 mL

Substrate Properties:
__

Format:
96-well Plate

Label or Dye:
__

Content And Storage:
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)

Form:
__

Product Line:
Pierce™

Substrate:
ONPG

Target Enzyme:
__

For Use With (Equipment):
Microplate Reader

Product Type:
Yeast beta-Galactosidase Assay Kit