PI75710
Thermo Scientific™ beta-Galactosidase Assay Reagent
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI75710-Each-of-1 | In Stock | ₹ 34,566.24 |
PI75710 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,566.24
GST (18%): ₹ 6,221.923
Total Price: ₹ 40,788.163
Assay
Reporter Enzyme
Detection Method
Colorimetric
For Use With (Application)
Absorbance
Quantity
3 x 25 mL
Substrate Properties
Chemical Substrate
Format
96-well Plate
Label or Dye
Enzyme Labeled
Content And Storage
Store at -20°C.
Form
Liquid
Product Line
Pierce™
Substrate
Beta-Gal Substrate
Target Enzyme
Beta-Galactosidase
For Use With (Equipment)
Microplate Reader
Product Type
Pierce beta-Galactosidase Assay Reagent
Related Products
Description
- Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit
- The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity
- Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution
- Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity
- With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles
- The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification
- Highlights: Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatiible with prepared lysates from other species such as bacteria Includes: Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No
- 78503), and ready-to-use Stop Solution
Compare Similar Items
Show Difference
Assay: Reporter Enzyme
Detection Method: Colorimetric
For Use With (Application): Absorbance
Quantity: 3 x 25 mL
Substrate Properties: Chemical Substrate
Format: 96-well Plate
Label or Dye: Enzyme Labeled
Content And Storage: Store at -20°C.
Form: Liquid
Product Line: Pierce™
Substrate: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
For Use With (Equipment): Microplate Reader
Product Type: Pierce beta-Galactosidase Assay Reagent
Assay:
Reporter Enzyme
Detection Method:
Colorimetric
For Use With (Application):
Absorbance
Quantity:
3 x 25 mL
Substrate Properties:
Chemical Substrate
Format:
96-well Plate
Label or Dye:
Enzyme Labeled
Content And Storage:
Store at -20°C.
Form:
Liquid
Product Line:
Pierce™
Substrate:
Beta-Gal Substrate
Target Enzyme:
Beta-Galactosidase
For Use With (Equipment):
Microplate Reader
Product Type:
Pierce beta-Galactosidase Assay Reagent
Assay: __
Detection Method: Reporter Enzyme
For Use With (Application): Absorbance
Quantity: 75 mL
Substrate Properties: __
Format: 96-well Plate
Label or Dye: __
Content And Storage: Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
Form: __
Product Line: Pierce™
Substrate: ONPG
Target Enzyme: __
For Use With (Equipment): Microplate Reader
Product Type: Yeast beta-Galactosidase Assay Kit
Assay:
__
Detection Method:
Reporter Enzyme
For Use With (Application):
Absorbance
Quantity:
75 mL
Substrate Properties:
__
Format:
96-well Plate
Label or Dye:
__
Content And Storage:
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
Form:
__
Product Line:
Pierce™
Substrate:
ONPG
Target Enzyme:
__
For Use With (Equipment):
Microplate Reader
Product Type:
Yeast beta-Galactosidase Assay Kit
Assay: __
Detection Method: __
For Use With (Application): __
Quantity: 25 Pre-cut Membranes
Substrate Properties: __
Format: Sheet
Label or Dye: __
Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Form: __
Product Line: __
Substrate: __
Target Enzyme: __
For Use With (Equipment): __
Product Type: __
Assay:
__
Detection Method:
__
For Use With (Application):
__
Quantity:
25 Pre-cut Membranes
Substrate Properties:
__
Format:
Sheet
Label or Dye:
__
Content And Storage:
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Form:
__
Product Line:
__
Substrate:
__
Target Enzyme:
__
For Use With (Equipment):
__
Product Type:
__
Assay: __
Detection Method: __
For Use With (Application): __
Quantity: 25 Pre-cut Membranes
Substrate Properties: __
Format: Sheet
Label or Dye: __
Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Form: __
Product Line: __
Substrate: __
Target Enzyme: __
For Use With (Equipment): __
Product Type: __
Assay:
__
Detection Method:
__
For Use With (Application):
__
Quantity:
25 Pre-cut Membranes
Substrate Properties:
__
Format:
Sheet
Label or Dye:
__
Content And Storage:
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Form:
__
Product Line:
__
Substrate:
__
Target Enzyme:
__
For Use With (Equipment):
__
Product Type:
__