TissueFab® bioink 

Description

• General description: 3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
• Application: Gelatin methacrylate-Alginate bioink formulation is derived from natural polymers - gelatin and alginate. Gelatin contains bioactive peptide sequences similar to native extracellular matrix which promotes integrin mediated cell adhesion and MMP sensitive enzymatic degradation which are essential for cellular functions such as migration, proliferation and differentiation. Alginate is a natural polysaccharide widely used in tissue engineering, serves as a viscosity enhancer in the bioink formulation. TissueFab®- GelAlg-Vis bioink formulation is optimized for 3D bioprinting of tissues and constructs using any extrusion based 3D bioprinter. TissueFab® - GelAlg-Vis bioink formulation can be used to bioprint cell-laden hydrogels in desired shape and can be crosslinked by a combination of chemical crosslinking and visible light mediated photocrosslinking for further culture and maturation of cells for tissue engineering and regenerative medicine applications. Gelatin methacrylate bioinks have been used for 3D bioprinting with high printing resolution, shape fidelity and cell viability[1][2]. Gelatin methacrylate based bioinks have been used to bioprint osteogenic[3], chondrogenic[2][4], hepatic[1][5][6], adipogenic[7], vasculogenic[8], epithelial[6], endothelial[9][10], cardiac valve[11], skin[12], tumor[10] and other tissues and constructs. Gelatin and alginate containing bioinks have been used for bioprinting of 3D constructs with various cell types including human mesenchymal stem cells (hMSC)[2][8], embryonic stem cells (ESC)[13], human umbilical vein endothelial cells (HUVEC)[8][10], fibroblasts[10], cancer cells[10] etc.
• Packaging: 10 mL in glass bottle
• Legal Information: TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany