TissueFab® bioink 

Description

• General description: TissueFab® bioink -(GelHep)MA Vis/405 nm, low endotoxin formulation can be used to bioprint cell-laden hydrogels in the desired shape without any supporting material. This bioink can be crosslinked in one step using exposure to 405 nm light for further culture and maturation of cells for tissue engineering and regenerative medicine applications.About the material: Heparin is a naturally occurring linear biopolymer and highly sulfated glycosaminoglycan (GAG). Research has demonstrated that heparin can modulate binding extracellular matrix proteins and sequester growth factors and cytokines, making them useful in 3D applications. The methacrylate-functionalization of heparin allows thermal or photochemical crosslinking via covalent conjugation. Heparins exhibit high anionic charge densities to promote large swelling ratios in water. Heparin-based bioinks are used in tissue engineering, 3D bio-printing, and drug delivery applications.Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation.Low Endotoxin, low bioburden: Endotoxins can negatively impact cellular growth, morphology, differentiation, inflammation, and protein expression. The bioburden is the number of contaminated organisms found in a given amount of material. We test each lot for endotoxins in addition to total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: Cell Culture FAQs: Bacterial Endotoxin Contamination
• Application: Currently, there is a need for high-quality, commercially available ready-to-use bioink formulations to enable the reproducible fabrication of synthetic tissues and organs by 3D Bioprintingdrug discoveryin-vitro disease modelsregenerative medicinecell-cultured meat
• Features and Benefits: Bioprinting models replicate biology for drug discovery and in vivo applicationsSterile, low endotoxinBatch control offers reproducible models for preclinical toxicology testing and drug screeningExtended shelf-life & stability
• Other Notes: Rethink your Bioinks with TissueFabReady-to-use formulations eliminate the lengthy bioink formulation development processProvide an optimized microenvironment conducive to the growth, proliferation, and maturation of cellsValidated with widely used cell types (including hMSCs) used in 3D BioprintingStep-by-step protocols developed and tested by internal R&D 3D Bioprinting ScientistsCompatible with different extrusion-based 3D bioprinter models
• Legal Information: TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany