Thermo Scientific™ Brilliance™ Salmonella Agar Base

Description

• Isolate and presumptively identify Salmonella species from food samples with chromogenic Thermo Scientific™ Brilliance™ Salmonella Agar Base (Dehydrated)
• An Inhibigen compound is comprised of two components, combined together by a bond that can only be cleaved by a specific enzyme
• When bound together, the inhibitor compound is not toxic and, therefore, can exist in a medium without harming micro-organisms
• Once inside the cell, the bond will be cleaved if the target enzyme is present
• When the bond is cleaved, the inhibitor molecule is released and disrupts cell wall synthesis, causing death of the organism
• As cells die and lyse, free inhibitor is released but cannot be taken up by other cells, resulting in targeted inhibition
• The Inhibigen in Brilliance Salmonella Agar targets Escherichia coli
• Novobiocin and cefsulodin, presented as a freeze-dried supplement (SR0194), are added to the medium to inhibit the growth of other competing flora such as Proteus spp
• and Pseudomonas spp
• Differentiation of Salmonella from the other organisms that grow on Brilliance Salmonella Agar is achieved through the inclusion of two chromogens that also target specific enzymes: caprylate esterase and β-glucosidase
• Caprylate esterase is an enzyme present in all samonellae as well as some species of Klebsiella , Enterobacter and Proteus
• Organisms possessing caprylate esterase cleave the chromogen to release an insoluble purple chromophore
• As the cells grow, the chromophore builds up and produces a purple-coloured colony
• Some Enterobacteriaceae, including Klebsiella and Enterobacter but not Salmonella , possess β-glucosidase2
• If these organisms grow, they will form blue or dark blue colonies, even if they are esterase positive, which make them easy to differentiate from purple Salmonella colonies
• Salmonella Selective Supplement, Part No
• SR0194E, is also available.