Thermo Scientific™ MacConkey Agar Base (Dehydrated)

Description

• Selectively cultivate gram-negative bacilli with Thermo Scientific™ Remel™ MacConkey Agar Base (Dehydrated)
• In 1900, MacConkey first described a neutral red bile salt medium for cultivation and identification of enteric organisms 1
• A detailed description of the selective and differential properties of the medium was published in 1905 2
• Over the years, MacConkey's original formula has been modified - the agar content has been reduced, the concentration of bile salts and neutral red has been adjusted, and sodium chloride has been added 3
• Selectively cultivate gram-negative bacilli with MacConkey Agar Base
• Selective: Crystal violet and bile salts are selective agents which inhibit most gram-positive organisms
• Ease of differentiation: Neutral red is an indicator which turns colonies pink when the carbohydrate is fermented
• This medium contains peptones that provide nitrogenous nutrients and amino acids essential for bacterial growth
• Bile salts and crystal violet are selective agents which inhibit gram-positive organisms
• MacConkey Agar Base is used with added carbohydrate to differentiate enteric gram-negative bacilli based on fermentation reactions
• When the carbohydrate is fermented, a local pH drop around the colony causes bile precipitation in the agar around the colony
• Agar is a solidifying agent
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.