Thermo Scientific™ Charcoal Agar Base (Dehydrated)

Description

• Selectively isolate Bordetella pertussis and Bordetella parapertussis with Thermo Scientific™ Remel™ Charcoal Agar Base (Dehydrated) supplemented with cephalexin
• Ensminger et al
• used a charcoal medium to grow B
• pertussis in vaccine production 1
• Regan and Lowe added 10% defibrinated horse blood to this agar supplemented with 40 μg/ml cephalexin and reported it to be an excellent enrichment and transport medium 2
• Horse blood is superior to sheep or human blood for recovery of Bordetella and ambient air provides more luxuriant growth of bordetellae than incubation in 5-10% CO 2 3,4
• This is a solid medium used to isolate Bordetella pertussis and Bordetella parapertussis which causes whooping cough and require special culturing techniques
• Cephalexin and Charcoal Agar Base provides selective isolation of Bordetella spp
• from respiratory specimens by inhibiting penicillin-resistant staphylococci, some coliforms and some strains of Haemophilus influenzae
• Growth factor: Horse blood supplies growth factors for B
• pertussis and neutralizes inhibitory substances
• Selective: Cephalexin provides selective isolation of Bordetella spp
• Charcoal Agar Base contains beef extract, peptones and nicotinic acid that provide essential nutrients for growth of Bordetella
• Starch and charcoal are added to absorb toxic fatty acids and peroxides
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.