Thermo Scientific™ Bile Esculin Azide Agar with Vancomycin

Description

• Isolate and presumptively identify vancomycin-resistant enterococci (VRE) from surveillance cultures with Thermo Scientific™ Remel™ Bile Esculin Azide Agar with Vancomycin
• Sodium azide and 1% oxgall are inhibitory to Gram-negative bacteria other than group D streptococci
• Vancomycin (6μg/mL) is added to select for resistant strains of enterococci
• Differentiation is facilitated by the hydrolysis of esculin in the presence of bile, resulting in the production of esculetin and dextrose
• Ferric ammonium citrate supplies ferric ions which react with esculitin to form a black-brown complex
• Members of the genus Enterococcus are normal residents of the gastrointestinal and biliary tracts 1,2
• Enterococci have become significant agents of human disease, largely because of their evolving resistance to antimicrobial agents
• VRE surveillance-screening has been found to facilitate earlier identification of colonized patients leading to more efficient containment of the organism
• Timely identification of VRE is improved with the use of the primary isolation medium for surveillance cultures
• Sahm et al
• demonstrated that Bile Esculin Azide Agar with 6μg/mL Vancomycin provides for rapid presumptive identification of VRE 3
• Selective : selective agents sodium azide and 1% oxgall are inhibitory to Gram-negative bacilli and most Gram-positive bacteria other than group D streptococci while vancomycin (6μg/mL) selects for resistant strains of enterococci
• Easy-to-read : vancomycin-resistant enterococci are easy to read due to brown-black complex surrounding the colonies Ready-to-use : convenience of prepared media
• Esculin in the medium allows for early recognition of presumptive enterococcal isolates, selective agents inhibit the growth of most commensal microbial flora, and vancomycin provides additional selectivity against vancomycin-sensitive and other Gram-positive organisms
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.