Thermo Scientific™ Modified EMB (HHT) Agar w/Lactose, Sucrose

Description

• Isolate and differentiate gram-negative enteric bacilli from clinical specimens with Thermo Scientific™ Remel™ Modified EMB (HHT) Agar w/Lactose, Sucrose
• Holt-Harris and Teague 1 developed Eosin Methylene Blue Agar (EMB Agar)
• The combination of dyes, lactose and sucrose incorporated in the medium provides a differential plating medium which helps to identify fermentative, gram-negative bacilli
• Modified (HHT) EMB Agar w/Lactose, Sucrose is a solid medium used to differentiate gram-negative enteric bacilli
• Ready- to-use: Convenience of prepared media Easy-to-read : Visible color change due to biochemical reaction Selective medium: Eosin and methylene blue dye selectively inhibit the growth of gram-positive organisms Conforms to CLSI standards 2 Modified (HHT) EMB Agar w/Lactose, Sucrose contains gelatin peptone that supplies amino acids and essential nutrients necessary for the growth of bacteria
• Lactose fermenting coliform and lactose nonfermenters are distinguished based on different colored colonies formed
• Eosin and methylene blue dyes also act as selective agents to inhibit the growth of gram-positive organisms
• The characteristic of certain coliforms to ferment lactose or sucrose can facilitate their identification and differentiation
• Eosin dye combines with methylene-blue indicator to produce a color change when lactose or sucrose is fermented
• Lactose fermenters such as Escherechia coli produce blue-black colonies with a green metallic sheen due to amide bonding of dye in an acidic environment
• Other coliforms form mucoid, pink-brown colonies in less acidic conditions, whereas lactose nonfermenters Shigella and Salmonella form transparent, colorless or amber colored colonies
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.