Thermo Scientific™ Middlebrook 7H10 Agar

Description

• Cultivate and isolate Mycobacterium species with Thermo Scientific™ Remel™ Middlebrook 7H10 Agar
• Based on the formulations developed initially by Dubos and Middlebrook 1 , and modified by Middlebrook and Cohn 2 , the Middlebrook 7H10 agar promotes the faster growth of Mycobacterium species and protects tubercle bacilli against a variety of toxic agents 1
• Middlebrook 7H10 Agar is a solid medium recommended for use in qualitative procedures for the cultivation and isolation of mycobacteria
• Mycobacteria are immobile, slow-growing, rod-shaped, gram-positive bacteria with high genomic content
• The genus includes pathogens known to cause severe diseases in mammals, including tuberculosis and leprosy
• Promotes: the faster growth of Mycobacterium Selective: Malachite-green dye is a selective agent, which partially inhibits bacteria other than mycobacteria
• Ready-to-use : the convenience of prepared media
• This medium contains inorganic salts which are essential to the growth of mycobacteria
• Glycerol is a source of carbon and energy
• Sodium citrate is converted to citric acid, which holds inorganic cations in solution
• The malachite-green dye is a selective agent, which partially inhibits bacteria other than mycobacteria
• Oleic Albumin Dextrose Catalase (OADC) Enrichment is a supplement which contains the following additives: sodium chloride to maintain osmotic equilibrium; dextrose, an energy source; catalase to destroy toxic peroxidases that may be present in the medium; oleic acid, which is required in the metabolism of mycobacterium; albumin to protect the tubercle bacilli against toxic agents
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.