Thermo Scientific™ RPMI 1640 Agar w/MOPS and 2% Glucose

Description

• Determine susceptibility to antifungal agents by testing Candida spp
• directly from colonies grown on non-selective media with Thermo Scientific™ Remel™ RPMI 1640 Agar w/MOPS and 2% Glucose
• RPMI 1640 was developed by Moore et al
• and is based on RPMI-1630 series of media utilizing a bicarbonate buffering system
• When supplemented with morpholinepropanesulfonic acid (MOPS), glucose and agar, RPMI-1640 has demonstrated accuracy for use with gradient-based systems with results comparable to that obtained with the CLSI reference method for testing Candida spp
• against antifungal agents 1-7
• Invasive fungal infections are of great clinical relevance and pose therapeutic challenges due to increasing resistance to the most commonly prescribed antifungals
• Concerns of emerging resistance and the introduction of new antifungal agents have led to increased demand for accurate species identification and antifungal susceptibility testing
• RPMI 1640 Agar w/MOPS and 2% glucose is a plated medium that is used with antibiotic gradient-based systems for susceptibility testing of Candida spp
• which causes nonsocomical bloodstream infections (BSI) in the United States 8,9
• RPMI-1640 medium is widely used in cell culture and also as a reference method for antifungal broth microdilution recommended by CLSI
• The use of RPMI-1640 medium supplemented with MOPS, agar, and glucose in the yeast susceptibility test in conjunction with antifungal gradient-based systems has been widely published and suggested as a reliable alternative to traditional broth dilution since the 1990s
• Easy-to-use – Preformed, stable antibiotic gradient strip is used
• Easy-to-read – After incubation a symmetrical inhibition ellipse centred along the carrier is seen and the zone edge intersects the strip at the MIC value given in μg/ml
• Results comparable to the Clinical and Laboratory Standards Institute (CLSI) reference method for antifungal broth microdilution
• RPMI 1640 Agar w/MOPS and 2% Glucose contains MOPs, glucose, RPMI-1640, agar and demineralized water
• This medium uses gradient-based systems
• The gradient method is based on a combination of the concepts of both dilution and diffusion tests, but differs from conventional disk methods
• When the strip is applied to the inoculated agar plate , there is an immediate release of the antibiotic into the agar matrix
• A continuous and exponential gradient of antibiotic concentration is created beneath the carrier
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.