Thermo Scientific™ Gelatin Medium (5mL)

Description

• Differentiate and Identify Enterobacteriaceae spp
• using Thermo Scientific™ Remel™ Gelatin Medium (Nutrient Gelatin)
• In their studies of gelatin liquefaction by gram-negative bacilli, Levine and Carpenter incorporated gelatin into the nutrient medium 1
• The production of gelatinase by some bacteria and the resulting liquefaction of gelatin can be used to differentiate certain bacilli
• Gelatin Medium (Nutrient Media) (12%) is recommended as a standard method for use in taxonomic work because the rate of gelatin liquefaction is important in the characterization of the Enterobacteriaceae 2
• Certain genera of enteric gram-negative bacilli and nonfermentive gram-negative bacilli can be differentiated and identified by the production of gelatinase
• Gelatinase is a proteolytic enzyme that hydrolyzes gelatin into its constituent amino acids with a loss of its gelling characteristics 3
• Ready to use – Convenience of prepared media
• Recommended as a standard method for use in taxonomic work because the rate of gelatin liquefaction is important in the characterization of the Enterobacteriaceae 2
• Easy to interpret - Positive test - Liquefacation after refrigeration Negative test - Solidification after refrigeration The medium contains peptone and beef extract that supply amino acids and other essential nutrients to support the growth of non-fastidious bacteria
• Gelatin is incorporated to determine an organism’s ability to produce gelatinase
• The proteolytic enzyme gelatinase decomposes gelatin into smaller components, resulting in liquefaction of the gelatin.