Thermo Scientific™ SF Broth

Description

• Differentiate enterococci from group D streptococci and other Streptococcus species with Thermo Scientific™ Remel™ SF Broth
• SF Broth was developed by Hajna and Perry to detect fecal streptococci in water, milk and other materials of sanitary importance 1
• Snyder and Lichstein had earlier demonstrated that sodium azide prevented swarming of Proteus and allows selective isolation of fecal streptococci from clinical samples
• Fecal streptococci consist of enterococci and group D streptococci of human origin that usually occur in higher densities in animal feces 2
• SF Broth is a liquid medium that is used to differentiate enterococci from group D streptococci and other Streptococcus species
• Enterococci grow in SF Broth and produce an acid (yellow) reaction, while other streptococci fail to grow and the medium remains purple in color
• Ready-to-use – Convenience of prepared media
• Bacteriostatic effect – Sodium azide is an inhibitor that exhibits bacteriostatic effect on gram-negative bacteria
• Easy-to-interpret – Turbidity and a yellow, yellow-brown color due to fermentation of dextrose indicates a positive test
• Negative test shows lack of turbidity and no color change i.e the medium remains purple
• SF Broth contains tryptone, dextrose, sodium chloride, dipotassium phosphate, monopotassium phosphate, sodium azide, brom cresol purple and demineralized water
• Tryptone and dextrose supply nutrients necessary to support the growth of streptococci
• Dextrose is the fermentable carbohydrate
• Sodium azide inhibits the cytochrome oxidase enzyme in the electron transport chain, thus exhibiting a bacteriostatic effect on gram-negative bacteria
• Brom cresol purple is the pH indicator
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.