Differentiate aerobic actinomycetes like Nocardia and Streptomyces using Thermo Scientific™ Remel™ Lysozyme Broth
A taxonomic scheme for identification of aerobic actinomycetes is developed based on biochemical characteristics which included testing for the growth in the presence of lysozyme 1
The enzyme lysozyme breaks down the cell walls of certain aerobic actinomycetes such as Streptomyces spp
and are referred sensitive and will not grow in lysozyme broth
Lysozyme resistance is more reliable to differentiate Nocardia and Streptomyces 2
The aerobic actinomycetes are a heterogenous group of genera and include species of Nocardia and Streptomyces 3
As a group, the organisms are gram-positive, catalase-positive and may be presumptively identified by staining properties, microscopic morphology, and substrate degradation 4
Lysozyme Broth contains lysozyme that inhibits the growth of some aerobic actinomycetes, but not all
Most Nocardia spp
are resistant and grow in the presence of lysozyme
This medium contains beef extract, peptone, and glycerol that supply carbohydrates, vitamins, nitrogen compounds, salts, and amino acids necessary for the growth of aerobic actinomycetes.