Thermo Scientific™ Urea Broth For AFB

Description

• Differentiate Mycobacterium species based on urea hydrolysis with Thermo Scientific™ Remel™ Urea Broth For AFB
• In 1979, Steadham developed the urease test to help in identification of mycobacteria 1
• This broth test helped to achieve more consistent results for urease activity
• The determination of urea hydrolysis (release of ammonia) helps in the characterization of mycobacterial strains, in particular, to distinguish scotochromogens and nonphotochromogens
• 2-5 Urea Broth For AFB is a liquid medium used to detect Mycobacterium species based on urea hydrolysis
• This is a highly buffered medium which helps in differentiation of mycobacterial strains after 7 days incubation
• Urea Broth For AFB contains peptone and dextrose that supply essential nutrients required for growth of mycobacteria
• Urea acts as the substrate for hydrolysis
• Polysorbate 80 is a surfactant which aids in the dispersion of aggregates
• The low pH of 5.8 facilitates a clear-cut differentiation of positive and negative results
• Phenol red (1%) serves as the pH indicator
• When urea is hydrolyzed ammonia is released and the resulting alkalinity is evidenced by the phenol red indicator
• A dark pink or red color development is observed in the case of a positive test whereas broth remains yellow in the case of a negative test
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.